Gold is the new silver! Silver enhancement has long been used by microscopists to better visualize gold labeling, especially when using small gold particles. With GoldEnhance™, gold ions are specifically deposited around the gold nanoparticle, growing it in size, and offering several advantages over silver.
For TEM, the 1.4 nm Nanogold® can be enlarged to 3-20 nm for clear visiblility, even at low magnifications. Using an initial small gold immunoprobe allows better penetration into tissues (up to 40 microns!) and better labeling of antigens. Then gold enhancement makes everything clearly visible.
The development time can be extended to deposit more gold and make the signal visible by bright field (or reflection) light microscopy. With a little more development time, the signal can be seen by the naked eye, and can be used with dot blots or Westerns.
Deposit metallic gold to enlarge ultrasmall gold nanoparticles for EM - Use it with Nanogold®!
Slow enhancement for an easy control of particle size
Great for SEM: Gold gives a much better backscatter signal than silver
Low background - No autonucleation for 40 minutes
Neutral pH for best ultrastructural preservation
Permanent staining: does not fade
Resistant to osmium tetroxide etching: may safely be used before osmium tetroxide staining with no need for gold toning or other protective treatments. Gold is stable in the oxidizing agent OsO4; silver would dissolve.
Compatible with physiological buffers and other halide solutions --these would precipitate silver.
Can be used for specimens on metal surfaces (e.g. cell culture substrates)
High density of enlarged particles for clear imaging.
Resistant to osmium tetroxide etching: may safely be used before osmium tetroxide staining with no need for gold toning or other protective treatments (silver is dissolved by the oxidizing agent OsO4; gold is stable).
for SEM, gold gives a much better backscatter signal than silver.